Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: Graphical representation of P. polymyxa identification by MALDI Biotyper Software. The colour of the peaks reflects the degree of matching of ( a ) ATCC 842 T , ( b , c ) DSM 292, and ( d , e ) DSM 365 with the reference MSPs (green = full match, yellow = partial match and red = mismatched) reported in the lower part of the graphs (blue spectra).

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques: Software

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: Mass spectra from 2000 to 15,000 Da of ATCC 842 T , DSM 292 and DSM 365 were processed and analysed by ClinProTools software. ( a ) Pseudo-gel like view of ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) mass spectra (15 replicates per class) displayed on a rainbow scale. Pseudo-gel like view of ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) mass spectra (15 replicates per class) displayed on a rainbow scale. The colour bar on the right side of the gel view indicates the peak intensity; 2-D PCA ( b ) and Loadings of 2D-PCA ( c ) plots of ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue).

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques: Software

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: Average spectra of the most characteristic peaks among P. polymyxa strains. Intensities of characteristic peaks in ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) are shown on y-axes and expressed in arbitrary intensity units.

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques:

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: Performance of classification models of ATCC 842 T , DSM 292 and DSM 365.

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques:

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: List of discriminative peaks according to QC and SNN classification models.

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques:

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model). The intensities of peaks were expressed in arbitrary intensity units and served as y-axes. The ellipses represent the 95% confidence intervals of peak intensity for each strain.

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques:

Journal: Scientific Reports

Article Title: MALDI-TOF as a powerful tool for identifying and differentiating closely related microorganisms: the strange case of three reference strains of Paenibacillus polymyxa

doi: 10.1038/s41598-023-50010-w

Figure Lengend Snippet: Comparison of main differing features in the phenotypical characterisation and fatty acids cellular composition of ATCC 842 T , DSM 292 and DSM 365.

Article Snippet: Figure 4 2D scatter plots of characteristic peaks for ATCC 842 T (red), DSM 292 (green) and DSM 365 (blue) selected by QC and SNN classification models. ( a ) Scatter plots of peaks 2944 and 3314 Da (selected by the QC model) and ( b ) 5504 and 9492 Da (selected by the SNN model).

Techniques: Comparison